PHCbi
PHC Corporation of North America, a Member of PHC Group Live Cell Metabolic Analyzer How To Use , How to use The Live Cell Metabolic Analyzer

How To Use

Live Cell Metabolic Analyzer

The LiCellMo offers these unique advantages:
  • real-time monitoring of glucose and lactate concentrations
  • continuous measurements
  • no sampling of the culture medium and use of the same cells for separate evaluation after measurement
 
 

The "Live Cell Metabolism Analyzer (LiCellMo)" can be used as follows:

 

1. Prepare sample and equipment

  1. 1.1Autoclave the Plate adapters and return the refrigerated Sensor module to the room temperature beforehand.
  2. 1.2Utilizing a standard 24-well plate, seed samples following your lab’s cell culture protocols.

2. Install sensor module

Assay and install sensor module in the biological safety cabinet.

  1. 2.1Once sample preparation is complete, place the 24-well plate into the plate adapter bottom and close the plate adapter top.
  2. 2.2After the sensor module is placed atop the preferred 24-well plate, it is ready to be placed into the Detector.

The electrochemical in-line sensors are inserted directly into the culture media and measure glucose and lactate concentrations continuously. Measuring glucose and lactate concentrations in real time enables the direct quantification of glycolytic activity and can be used to calculate cellular metabolic rate.

3. Place the detector

  1. 3.1Set the sensor module assembly on the tray of detector. The detector is housed in the chamber of a CO2 incubator so that glycolytic changes can be evaluated directly.

4. Assay and data analysis from the controller

Set up and start measurement from controller.
Data during continuous measurement is confirmed from the controller's display, allowing analysis while maintaining the cell culture condition without sampling cells.

・Create an assay template

Edit an assay template based on your experimental design. Enter information such as cell and medium, and well layout in the assay template. To edit an assay template, glucose and lactate concentrations of the medium to be measured (measured medium) is required.

Grouping of cultured cells in the well plate

Press the Edit button for any Group and enter the Group information.

Name Enter the name of the Group (optional).
Cell Enter the name of the cell to be measured (optional).
Medium Enter the name of the measured medium (optional).
Calib. A Glc/Lac
Calib. B Glc/Lac		 Enter the glucose and lactate concentrations of the Calibration A solution and B solutions (required).

・Data analysis

Analyze data of project of which assay has completed. Smoothing is performed to remove noise included in the measured values. By differentiating the smoothed data, the glucose consumption rate and lactate production rate are calculated.

Data analysis and saving analysis results

Press the Analyze button to perform analysis. Analysis results (smoothed concentration/metabolic rate) are displayed in the graph area.

Calculation of metabolic rate | Analysis

Calculate the metabolic rate of each group from the concentration change.

Data Select the data types to display in the graph area.
Sensor Select the sensor types to be displayed in the graph area with the check boxes. Use the Glc/Lac button to switch between thick line display and normal line width display.
Group Use the check boxes to select the group types to be displayed in the graph area.
Statistics Tab Display the mean and standard deviation of concentration and metabolic rate for each Group.
Graph option Use the check box to switch display/hide of the unbiased standard deviation of smoothed concentration and metabolic rate of each group.

Screen capture

After saving the analysis result by pressing the Save Result button, you will be able to save the screen capture image.
The captured image is stored in a form linked to the analysis result, and data can be export.

  1. 1Press the Save Result button to save the analysis result.
  2. 2Press the camera button to save the screen capture image. The number on the top right of the camera button indicates the number of images saved.

5. Exporting measurement results

Measurement data and analysis results can be output to a flash drive.